Microarray Data Analysis
Spots are analyzed by the adaptive quantification method with local background subtractions
Copy tabulated data to the textarea as shown. First valid data must correspond to Column 1/Row 1.
All lines preceding data for Column 1/Row 1 will be removed previous to calculations.
Computational procedure: 1. For each spot, the following is computed for both channels (1 and 2): (value-background)*100/sum(value-background)) 2. With previous results, ch1/ch2 and ch2/ch1 are computed 3. As there are several replicas for ch1/ch2 and ch2/ch1, median and its base 10 logarithm is computed. 4. In a table, medians and logs are represented. A color code is used to show over-expression (red) or under-expression (blue).